LOCUS       sequence-104369-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Duet plasmid for weak split mCherry assembly (as compared to 
            N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
  TITLE     Designed Phosphoprotein Recognition in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. 
            Epub 2014 Oct 6.
  PUBMED    25272187
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500658w";
            journalName: "ACS Chem Biol"; date: "2014-11-21- 21"; volume: "9"; 
            issue: "11"; pages: "2502-7"
COMMENT     Sequence Label: pNAS1B split mCherry N-MESVD/TRAP(RK)-C
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(103..121,122..127,128..144,145..150,151..176)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    128..146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             186..197
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(355..373)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     623..642
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      765..851
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
       61 aaatccctta acgtgagttt tcgttccact gagcgaagat cttccctatc agtgatagag
      121 attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca ctgaccgaat
      181 tcattaaaga ggagaaaggt accatggcat ccgtgagcaa gggcgaggag gataacatgg
      241 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      301 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      361 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      421 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      481 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      541 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      601 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      661 cctccgagcg gatgtacccc gaggacggtg gctctggctc tgggtcgact ggtatggaaa
      721 gcgtggatta atgaaagctt catggtacgc gtgctgccag gcatcaaata aaacgaaagg
      781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      841 gtaggacaaa tcc
//
