LOCUS       sequence-104412-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER targeted transmembrane domain with monomeric EGFP oriented 
            towards the cytoplasm. Used for assessing fluorescent protein 
            oligomerization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Costantini LM, Fossati M, Francolini M, Snapp EL
  TITLE     Assessing the tendency of fluorescent proteins to oligomerize under 
            physiologic conditions.
  JOURNAL   Traffic. 2012 May;13(5):643-9. doi: 
            10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20.
  PUBMED    22289035
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01336.x"; journalName: "Traffic"; date: 
            "2012-05"; volume: "13"; issue: "5"; pages: "643-9"
COMMENT     Sequence Label: CyTERM-mGFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(218..220,221..223,224..736)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(263..284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(524..543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctggta ccccttgcag cttccagttg
       61 tcaggaagag aaggctccca tggatcctgt ggtggtgctg gggctctgtc tctcctgttt
      121 gcttctcctt tcactctgga aacagagcta tgggggaggg aagcttcgaa ttctgcagtc
      181 gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
      241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      661 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      721 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgcc
//
