LOCUS       sequence-104522-        7836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast plasmid with Hsp82.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Jiang L, Mishra P, Hietpas RT, Zeldovich KB, Bolon DN
  TITLE     Latent effects of Hsp90 mutants revealed at reduced expression 
            levels.
  JOURNAL   PLoS Genet. 2013 Jun;9(6):e1003600. doi: 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27.
  PUBMED    23825969
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013-06"; volume: "9(6):e1003600. doi"; pages: " 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27"
COMMENT     Sequence Label: p414GPD_Hsp82_CycTER
FEATURES             Location/Qualifiers
     source          1..7836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..27)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2754..2774
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2799..3442
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3336..3359
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3470..3487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5666..5682)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5667..5683)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5685..5932
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5701..5719)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5950..5969)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5951..5969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5979..5996)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5979..5995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5988..6010)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6140..6595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6227..6246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6437..6458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        complement(7370..7650)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(7667..7686)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7786..7808
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       61 ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tacctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcc tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     2761 accctcacta aagggaacaa aagctggagc tcagtttatc attatcaata ctcgccattt
     2821 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
     2881 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
     2941 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
     3001 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
     3061 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
     3121 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
     3181 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
     3241 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
     3301 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     3361 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcatttt tttagtttta
     3421 aaacaccaga acttagtttc gagaaaaata gaatactagt atgggtggtc accaccatca
     3481 ccaccacggt ggtcatatgg ctagtgaaac ttttgaattt caagctgaaa ttactcagtt
     3541 gatgagtttg atcatcaaca ccgtctattc taacaaggaa attttcttga gagaactgat
     3601 atctaatgcc tcggatgcgt tggataaaat tagatacaaa tctttgtctg atccaaagca
     3661 attggaaaca gaaccagatc tctttattag aatcactcca aagccagagc aaaaagtttt
     3721 ggaaatcaga gattctggta ttggtatgac caaggctgaa ttgattaata acttgggtac
     3781 cattgccaag tctggtacca aagccttcat ggaagctcta tctgctggtg ccgatgtatc
     3841 catgattggt caattcggtg ttggttttta ctctttattc ttagttgccg acagagttca
     3901 ggttatttca aagagcaacg acgacgaaca atacatctgg gaatccaacg ctggtggttc
     3961 tttcactgtt actctagacg aagttaatga aagaattggt aggggtacca tcttgaggtt
     4021 attcttgaaa gatgaccaat tggagtactt ggaagaaaag agaataaagg aagttatcaa
     4081 gagacattct gagttcgtgg cctacccaat ccaattagtc gtcaccaagg aagttgaaaa
     4141 ggaagttcca attccagaag aagaaaagaa agacgaggaa aagaaggatg aggaaaagaa
     4201 ggatgaagac gacaagaaac caaaactcga ggaagtcgat gaagaagagg aaaagaagcc
     4261 aaagacgaaa aaagttaaag aagaagttca agagatagaa gaactaaaca agactaagcc
     4321 tttgtggact agaaacccat ctgatatcac tcaagaagaa tacaatgctt tctataagtc
     4381 tatttcaaac gactgggaag acccattgta cgttaagcat ttctccgttg aaggtcaatt
     4441 ggaatttaga gctatcttat tcattccaaa gagagcacca ttcgacttgt ttgagagtaa
     4501 aaagaagaag aataatatca agttgtacgt tcgtcgtgtt ttcatcactg atgaagctga
     4561 agacttgatt ccagagtggt tatctttcgt caagggtgtt gttgactctg aggatttacc
     4621 attgaatttg tccagagaaa tgttacaaca aaataagatc atgaaggtta ttagaaagaa
     4681 cattgtcaaa aagttgattg aagccttcaa cgaaattgct gaagactctg aacaatttga
     4741 aaagttctac tcggctttct ccaaaaatat caagttgggt gtacatgaag atacccaaaa
     4801 cagggctgct ttggctaagt tgttacgtta caactctacc aagtccgtag atgagttgac
     4861 ttccttaact gattacgtta ccagaatgcc agaacaccaa aagaacatct actacatcac
     4921 tggtgaatct ctaaaggctg tcgaaaagtc tccatttttg gatgccttga aggctaaaaa
     4981 cttcgaggtt ttgttcttga ccgacccaat tgatgaatac gccttcactc aattgaagga
     5041 attcgaaggt aaaactttgg ttgacattac taaagatttc gaattggaag aaactgacga
     5101 agaaaaagct gaaagagaga aggagatcaa agaatatgaa ccattgacca aagctttgaa
     5161 agaaattttg ggtgaccaag tggagaaagt tgttgtttct tacaaattgt tggatgcccc
     5221 agctgctatc agaactggtc aatttggttg gtctgctaac atggaaagaa tcatgaaggc
     5281 tcaagccttg agagactctt ccatgtcctc ctacatgtct tccaagaaga ctttcgaaat
     5341 ttctccaaaa tctccaatta tcaaggaatt gaaaaagaga gttgacgaag gtggtgctca
     5401 agacaagact gtcaaggact tgactaagtt attatatgaa actgctttgt tgacttccgg
     5461 cttcagtttg gacgaaccaa cttcctttgc atcaagaatt aacagattga tctctttagg
     5521 cctgaacatt gatgaggatg aagaaacaga gactgctcca gaagcatcca ccgcagctcc
     5581 ggttgaagag gttccagctg acaccgaaat ggaagaggta gattaatgga tcccccgggc
     5641 tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgagtcatgt aattagttat
     5701 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag
     5761 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
     5821 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
     5881 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta
     5941 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6001 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6061 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6121 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6541 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcctga
     6601 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggc aagtgcacaa
     6661 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
     6721 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
     6781 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
     6841 cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca
     6901 atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg
     6961 tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat
     7021 aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac
     7081 gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa
     7141 cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta caagacttga
     7201 aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc
     7261 cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct gcaagccgca
     7321 aactttcacc aatggaccag agactacctg tgaaattaat aacagacata ctccaagctg
     7381 cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt
     7441 ggccctctcc ttttcttttt tcgaccgaat taattcttaa tcggcaaaaa aagaaaagct
     7501 ccggatcaag attgtacgta aggtgacaag ctatttttca ataaagaata tcttccacta
     7561 ctgccatctg gcgtcataac tgcaaagtac acatatatta cgatgctgtc tattaaatgc
     7621 ttcctatatt atatatatag taatgtcgtt tatggtgcac tctcagtaca atctgctctg
     7681 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7741 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7801 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
