LOCUS       sequence-104591-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            LSSmOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-LSSmOrange-CNA35
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagc
       61 aatgccttac aggtcgactg aagccttggt atctttatcc tgttttaaaa cttttaattc
      121 accttttaca gtaccttcaa taccggcatt agcattaata ttgtgcacag tatgattaaa
      181 tgatttcccg ttcacttctt ccttaccatg ctcttgatac caagcttgtg aattattaac
      241 aaactctttt tgctgttcat tcgtaatttt ggttttgtag ttaattgaaa aactattata
      301 tgacccatag ccttgtggaa ttgttacatc aattgtgttc ttcgtattat caacagttat
      361 tttagaacct ggaaaggctt tttcaaaatc agtaattgca ctttgtccac tataataatt
      421 gctatgtgta cctgtcacat taatgtttaa tgtgcttaaa tctaactgct gtccaccttg
      481 aatctgatcc tttatagtaa tatctttcga tacataactt ttttcattgt taatatttaa
      541 aaaccatcgt acatgtgtcg tatcttctgg tagcatatct cccgttttat aatagaaaac
      601 actacttgtt cccgcttcac ttttatgaac cgtaacattc gttgatttat tcccagatgt
      661 tatcgtagct actttgtcat ctgaagtatt tgtttgcgtt aaatttcttc cttgtacttc
      721 aaattctgca aatcccgaaa catcacttaa tttttctact ttatcattga atgtaattgt
      781 tgcaccgtct ggtgtaataa ctgcttgacc cacctgttca cctttaacag ttaatggtac
      841 tgttttacta taaccctcta tctttactgt accgcttgtc ggccatgcca ctttaatcat
      901 gtcaccattt tgtatttttc catttttatc gtcgaacgtc atttttactg tcgttttacc
      961 accatcttct atcttagacg gtgatacagt taaatctgta acattcgttg atgaaatatc
     1021 tcgtgcggat ccgtggaatt ctttatacag ttcgtccata ccgccagtag aatgacggcc
     1081 ctcggcacgt tcgtactgtt caacaatc
//
