LOCUS       sequence-104592-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            LSSmOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-LSSmOrange-CNA35
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatggtg agcaaaggtg
      241 aggaaaacaa tatggcaatc attaaagagt ttatgcgttt caaggtccgt atggaaggtt
      301 ctgtgaacgg tcacgagttc gagattgaag gcgagggtga gggtcgtccg tatgagggct
      361 tccagactgt taaactgaag gtgaccaaag gcggtcctct gccgttcgcc tgggatatcc
      421 tgtctccgca attcacatat gggtccaagg cgtatgttaa acaccctgca gatatccccg
      481 attatttaaa actgtcattc ccggaaggct tcaaatggga gcgtgtaatg aacttcgagg
      541 acggcggcgt cgtaaccgtt acccaggact cctctctgca ggacggtgag tttatttaca
      601 aagtgaagct gcgcggcact aactttccgt ctgacggtcc ggttatgcag aaaaagacta
      661 tgggcatgga agcttcctct gaacgtatgt atccggaaga cggcgcgctg aaaggtgagg
      721 acaaactgcg tctgaagctg aaagatggcg gtcattacac ctctgaagtt aaaaccacct
      781 acaaagccaa aaagccggta caactgccgg gtgcttacat tgtagatatt aaactggata
      841 tcacgagcca taacgaggat tacacgattg ttgaacagta cgaacgtgcc gagggccgtc
      901 attctactgg cggtatggac gaactgtata aagaattcca cggatccgca cgagatattt
      961 catcaacgaa tgttacagat ttaactgtat caccgtctaa gatagaagat ggtggtaaa
//
