LOCUS       sequence-104653-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with mKO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_mKO2
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..683)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
ORIGIN
        1 gcgcacattt ccccgaattg gtcgactgag taatgagcta ctgcatcttc tacctgctca
       61 gtaatgttgc cttcggtttt cctgacgagg cgatggccga tgtaatggtc tcctggcatt
      121 tcaagaatct cttttgccgc cttgtaagta gtcttcattt ggcatttgtg attgccgcct
      181 ccttcaagtt ttaggtacat cgtaacatca cccttcagaa ctccgtcgct ggcagtaatt
      241 ttctcggttg atggctccca atcaacactt tggttttgca tgataggacc atcggcagga
      301 aagttaaccc cagtaaattt ggatttgtgg tagaaggtgt ttcctctaag gcttatatgc
      361 gcactgactg aagcggaccc accatcttcg aactccaacg acctttccca tgacaggcct
      421 tcaggaaatg cttgtttgaa atagtctggt atctcttctg gatatttagt aaatactctg
      481 tggccgtaac agaacacgtg tgacactaag tcaaacgcga aaggcattgg cccgccctcg
      541 gccattgtga cgcgcagtgt catctcttga tgtccctcgt aaggtctgcc tgtgccttca
      601 ccttcaattg tgaactcatg cccattgacg gagccgtcca tgtagtacct catcttcatc
      661 tctggtttaa tcacactcac catctcgagg ttacgcgttt gatatcatg
//
