LOCUS       sequence-104689-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with minCMV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_minCMV
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(238..262)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        335..439
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(658..677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtatataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatggtcg accagctgag atctgaattc tccaggcgat
      241 ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac gccacctcga
      301 catactcgag ttacgcgtaa ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      361 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      421 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      481 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      541 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      601 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      661 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      721 ctattctcag
//
