LOCUS       sequence-104724-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with rtTA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_rtTA3
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..145)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gaattgtcga cagatcctta cttagttacc cggggagcat gtcaaggtca aaatcgtcaa
       61 gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt
      121 ctaagtcaaa atcgtcaagg gcgtcggtcg gcccgccgct ttcgcacttt agctgtttct
      181 ccaggccaca tatgattagt tccaggccga aaaggaaggc aggttcggct ccctgccggt
      241 cgaacagctc aattgcttgt ctcagaagtg ggggcataga atcggtggta ggtgtctctc
      301 tttcctcttt tgctacttga tgctcctgtt cctccaatac gcagcccagt gtaaagtggc
      361 ccacggcgga cagagcgtac agtgcgttct ccagggagaa gccttgctga cacaggaacg
      421 cgagctgatt ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg agatgcactt
      481 tagccccgtc gcgatgtgag aggagagcac agcggtatga cttggcgttg ttccgcagaa
      541 agtcttgcca tgactcgcct tccagggggc agaagtgggt atgatgcctg tccagcatct
      601 cgattggcag ggcatcgagc agggcccgct tgttcttcac gtgccagtac agggtaggct
      661 gctcaactcc cagcttttga gcgagtttcc ttgtcgtca
//
