LOCUS       sequence-104746-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Gag and Pol; 
            also requires pRSV-Rev (Addgene#12253) and envelope expressing 
            plasmid (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Nov . 72(11):8463-71."
COMMENT     Sequence Label: pMDLg/pRRE
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          150..625
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      703..712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc ccctcgaagc ttacatgtgg
      121 taccgagctc ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
      181 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
      241 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
      301 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
      361 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
      421 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
      481 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
      541 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
      601 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
      661 tcactttggc aaagcacgtg agatctgaat tcgagatctg ccgccgccat gggtgcgaga
      721 gcgtcagtat taagcggggg agaattagat cgatgggaaa aaattcggtt aaggccaggg
      781 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      841 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      901 caaccatccc ttcagacagg atcagaaga
//
