LOCUS       sequence-104802-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::rtTA3-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::rtTA3-bGHpA
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    12..236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(266..376)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gaattggtcg accatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
       61 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
      121 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
      181 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagctcg
      241 acagatcctt acttagttac ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag
      301 caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa
      361 aatcgtcaag ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac
      421 atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct
      481 caattgcttg tctcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt
      541 ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg
      601 acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat
      661 tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttag
//
