LOCUS       sequence-10518-e        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector encoding human p53 with a 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW
  TITLE     Different mutant/wild-type p53 combinations cause a spectrum of 
            increased invasive potential in nonmalignant immortalized human 
            mammary epithelial cells.
  JOURNAL   Neoplasia. 2008 May . 10(5):450-61.
  PUBMED    18472962
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2008 May . 10(5):450-61."
COMMENT     Sequence Label: pLenti6/V5-p53_wt p53
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..1315
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
ORIGIN
        1 cggngggnca ntagcagagc tggttagtga accgtcagat cgcctggaga cgccatccac
       61 gctgttttga cctccataga agacaccgac tctagaggat ccactagtcc agtgtggtgg
      121 aattgatccc ttcaccatgg aggagccgca gtcagatcct agcgtcgagc cccctctgag
      181 tcaggaaaca ttttcagacc tatggaaact acttcctgaa aacaacgttc tgtccccctt
      241 gccgtcccaa gcaatggatg atttgatgct gtccccggac gatattgaac aatggttcac
      301 tgaagaccca ggtccagatg aagctcccag aatgccagag gctgctcccc gcgtggcccc
      361 tgcaccagca gctcctacac cggcggcccc tgcaccagcc ccctcctggc ccctgtcatc
      421 ttctgtccct tcccagaaaa cctaccaggg cagctacggt ttccgtctgg gcttcttgca
      481 ttctgggaca gccaagtctg tgacttgcac gtactcccct gccctcaaca agatgttttg
      541 ccaactggcc aagacctgcc ctgtgcagct gtgggttgat tccacacccc cgcccggcac
      601 ccgcgtccgc gccatggcca tctacaagca gtcacagcac atgacggagg ttgtgaggcg
      661 ctgcccccac catgagcgct gctcagatag cgatggtctg gcccctcctc agcatcttat
      721 ccgagtggaa ggaaatttgc gtgtggagta tttggatgac agaaacactt ttcgacatag
      781 tgtggtggtg ccctatgagc cgcctgaggt tggctctgac tgtaccacca tccactacaa
      841 ctacatgtgt aacagttcct gcatgggcgg catgaaccgg aggcccatcc tcaccatcat
      901 cacactggaa gactccagtg gtaatctact gggacggaac agctttgagg tgcgtgtttg
      961 tgcctgtcct gggagagacc ggcgcacaga ggaagagaat ctccgcaaga aaggggagcc
     1021 tcaccacgag ctgcccccag ggagcactaa gcgagcactg cccaacaaca ccagctcctc
     1081 tccccagcca aagaagaaac cactggatgg agaatatttc acccttcaga tccgtgggcg
     1141 tgagcgcttc gagatgttcc gagagctgaa tgaggccttg gaactcaagg atgcccaggc
     1201 tgggaaggag ccagggggga gcagggctca ctccagccac ctgaagtcca aaaagggtca
     1261 gtctacctcc cgccataaaa aactcatgtt caagacagaa gggcctgact cagacaaggg
     1321 ctcgagtcta gagggcncgc ntgaatc
//
