LOCUS       sequence-105687-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV zinc finger donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAVS1 SA-2A-puro-pA donor
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    147..676
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cggcgcgaat cgcccttaga gcagagccag gaacccctgt agggaagggg caggagagcc
       61 aggggcatga gatggtggac gaggaagggg gacagggaag cctgagcgcc tctcctgggc
      121 ttgccaagga ctcaaaccca gaagcccaga gcagggcctt agggaagcgg gaccctgctc
      181 tgggcggagg aatatgtccc agatagcact ggggactctt taaggaaaga aggatggaga
      241 aagagaaagg gagtagaggc ggccacgacc tggtgaacac ctaggacgca ccattctcac
      301 aaagggagtt ttccacacgg acacccccct cctcaccaca gccctgccag gacggggctg
      361 gctactggcc ttatctcaca ggtaaaactg acgcacggag gaacaatata aattggggac
      421 tagaaaggtg aagagccaaa gttagaactc aggaccaact tattctgatt ttgtttttcc
      481 aaactgcttc tcctcttggg aagtgtaagg aagctgcagc accaggatca gtgaaacgca
      541 ccagacagcc gcgtcagagc agctcaggtt ctgggagagg gtagcgcagg gtggccactg
      601 agaaccgggc aggtcacgca tccccccctt ccctcccacc ccctgccaag ctctccctcc
      661 caggatcctc tctggctcca tcgtaagcaa accttagagg ttctggcaag gagagagatg
      721 gctccaggaa atgggggtgt gtcaccagat aaggaatctg cctaacagga ggtgggggtt
      781 agacccaata tcaggaga
//
