LOCUS       sequence-105717-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, 
            Tan SM, Hide W, Lieberman J
  TITLE     miR-200 promotes the mesenchymal to epithelial transition by 
            suppressing multiple members of the Zeb2 and Snail1 transcriptional 
            repressor complexes.
  JOURNAL   Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69.
  PUBMED    25798844
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2015 Mar 23. doi: 10.1038/onc.2015.69."
COMMENT     Sequence Label: psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1028..1036
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gaacaccgag cgaccctgca gcgacccgct taaaagcttg gcattccggt actgttggta
       61 aagccaccat ggccgatgct aagaacatta agaagggccc tgctcccttc taccctctgg
      121 aggatggcac cgctggcgag cagctgcaca aggccatgaa gaggtatgcc ctggtgcctg
      181 gcaccattgc cttcaccgat gcccacattg aggtggacat cacctatgcc gagtacttcg
      241 agatgtctgt gcgcctggcc gaggccatga agaggtacgg cctgaacacc aaccaccgca
      301 tcgtggtgtg ctctgagaac tctctgcagt tcttcatgcc agtgctgggc gccctgttca
      361 tcggagtggc cgtggcccct gctaacgaca tttacaacga gcgcgagctg ctgaacagca
      421 tgggcatttc tcagcctacc gtggtgttcg tgtctaagaa gggcctgcag aagatcctga
      481 acgtgcagaa gaagctgcct atcatccaga agatcatcat catggactct aagaccgact
      541 accagggctt ccagagcatg tacacattcg tgacatctca tctgcctcct ggcttcaacg
      601 agtacgactt cgtgccagag tctttcgaca gggacaaaac cattgccctg atcatgaaca
      661 gctctgggtc taccggcctg cctaagggcg tggccctgcc tcatcgcacc gcctgtgtgc
      721 gcttctctca cgcccgcgac cctattttcg gcaaccagat catccccgac accgctattc
      781 tgagcgtggt gccattccac cacggcttcg gcatgttcac caccctgggc tacctgattt
      841 gcggctttcg ggtggtgctg atgtaccgct tcgaggagga gctgttcctg cgcagcctgc
      901 aagactacaa aattcagtct gccctgctgg tgccaaccct gttcagcttc ttcgctaaga
      961 gcaccctgat cgacaagtac gacctgtcta acctgcacga gattgcctct ggcggcgccc
     1021 cactgtctaa ggaggtgggc gaagccgtgg ccaagcgctt tcatctgcca ggcatccgcc
     1081 agggctacgg cctgaccga
//
