LOCUS       sequence-105818-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes nuclear dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-hR
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        121..139
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     121..138
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(194..227)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(235..268)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(277..310)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      324..333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatgcagagc aaaacaggaa gttgactcca gatggtcaca tgcttgctga aacgcctctc
       61 cctggcagca gtgcaattct aacagtttcc ctttctttta cagttcgcca tcctaggcct
      121 atttaggtga cactatagaa gagtacaagc tacttgttct ttttgcagga tccgccacca
      181 tgactagttc gagataactt cgtatagtat accttatacg aagttatcga gctcataact
      241 tcgtatagga tactttatac gaagttataa ctcgagataa cttcgtatag catacattat
      301 acgaagttat accggcagat ctcgccacca tggtgagtgt gattaaacca gagatgaaga
      361 tgaagtactt catggacggc tccgtcaatg ggcatgagtt cacagttgaa ggtgaaggca
      421 caggcaaacc ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg
      481 ggccaatgcc tttctcgttt gacttagtgt cacacacgtt ctgttacggc cacagacctt
      541 ttactaaata tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt
      601 catgggaaag gtcgttgcag ttcgtgggct gggaggcctc ctccgagcgg atgtaccccg
      661 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      721 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgc
//
