LOCUS       sequence-105939-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (OsUBI promoter) for direct cloning of 
            artificial microRNAs into rice MIR390 precursor. To use for 
            expressing amiRNAs in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pH7WG2B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(147..171)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
       61 acacagccag tctgcacctc gacggtctca catggtttgt tcttaccaca cgaccaatta
      121 aatcgagctc aagggtgggc gcgccgaccc agctttcttg tacaaagtgg tgatatcccg
      181 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
      241 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
      301 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
      361 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcgacg
      421 tcgggccctc tagaggatcc ccgggtaccg tgcagcgtcg cgtcgggcca agcgaagcag
      481 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
      541 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
      601 cggcaggcgg cctcctcctc ctctcacggc accggcagct ac
//
