LOCUS       sequence-105942-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..644)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(128..148)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     625..644
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cttcggtcgg agccatgggg tcgtgcgctc ctttcggtct ccggctaggc accgggcttg
       61 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
      121 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
      181 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
      241 tggtggtcgg gcgacacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
      301 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
      361 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
      421 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
      481 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg gtcgacggtg
      541 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc ccgggggacg
      601 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggttgt ggccatatta
      661 tcatcgtgtt tttc
//
