LOCUS       sequence-106117-        1302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes E. coli biotin ligase gene (BirA) for expression in yeast 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Rodgers ML, Paulson J, Hoskins AA
  TITLE     Rapid isolation and single-molecule analysis of ribonucleoproteins 
            from cell lysate by SNAP-SiMPull.
  JOURNAL   RNA. 2015 May;21(5):1031-41. doi: 10.1261/rna.047845.114. Epub 2015 
            Mar 24.
  PUBMED    25805862
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.047845.114"; journalName: "RNA"; date: "2015-05"; 
            volume: "21"; issue: "5"; pages: "1031-41"
COMMENT     Sequence Label: pVTU_260_BirA
FEATURES             Location/Qualifiers
     source          1..1302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             224..241
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             248..268
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             278..1243
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             278..1240
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 attcacgcac actactctct aatgagcaac ggtatacggc cttccttcca gttacttgaa
       61 tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat aaatagacct gcaattatta
      121 atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca
      181 atatttcaag ctataccaag catacaatca actccaagct atgcaccatc accatcacca
      241 tgctagcgag aatctttatt ttcagggcgc catgggtatg aaggataaca ccgtgccact
      301 gaaattgatt gccctgttag cgaacggtga atttcactct ggcgagcagt tgggtgaaac
      361 gctgggaatg agccgggcgg ctattaataa acacattcag acactgcgtg actggggcgt
      421 tgatgtcttt accgttccgg gtaaaggata cagcctgcct gagcctatcc agttacttaa
      481 tgctaaacag atattgggtc agctggatgg cggtagtgta gccgtgctgc ctgtgattga
      541 ctccacgaat cagtaccttc ttgatcgtat cggagagctt aaatcgggcg atgcttgcat
      601 tgcagaatac cagcaggctg gccgtggtcg ccggggtcgg aaatggtttt cgccttttgg
      661 cgcaaactta tatttgtcga tgttctggcg tctggaacaa ggcccggcgg cggcgattgg
      721 tttaagtctg gttatcggta tcgtgatggc ggaagtatta cgcaagctgg gtgcagataa
      781 agttcgtgtt aaatggccta atgacctcta tctgcaggat cgcaagctgg caggcattct
      841 ggtggagctg actggcaaaa ctggcgatgc ggcgcaaata gtcattggag ccgggatcaa
      901 catggcaatg cgccgtgttg aagagagtgt cgttaatcag gggtggatca cgctgcagga
      961 agcggggatc aatctcgatc gtaatacgtt ggcggccatg ctaatacgtg aattacgtgc
     1021 tgcgttggaa ctcttcgaac aagaaggatt ggcaccttat ctgtcgcgct gggaaaagct
     1081 ggataatttt attaatcgcc cagtgaaact tatcattggt gataaagaaa tatttggcat
     1141 ttcacgcgga atagacaaac agggggcttt attacttgag caggatggaa taataaaacc
     1201 ctggatgggc ggtgaaatat ccctgcgtag tgcagaaaaa taaggatcca gctttggact
     1261 tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cg
//
