LOCUS       sequence-106172-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mtZFN2G architecture bearing (-) FokI domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Gammage PA, Rorbach J, Vincent AI, Rebar EJ, Minczuk M
  TITLE     Mitochondrially targeted ZFNs for selective degradation of 
            pathogenic mitochondrial genomes bearing large-scale deletions or 
            point mutations.
  JOURNAL   EMBO Mol Med. 2014 Apr;6(4):458-66. doi: 10.1002/emmm.201303672. 
            Epub 2014 Feb 24.
  PUBMED    24567072
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/emmm.201303672"; journalName: "EMBO Mol Med"; date: 
            "2014-04"; volume: "6"; issue: "4"; pages: "458-66"
COMMENT     Sequence Label: pcDNA3.1(-)_mtZFN(-)2G
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             229..252
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaacg ggccctctag aatgttgggg tttgtgggtc gggtggccgc tgctccggcc
      121 tccggggcct tgcggagact caccccttca gcgtcgctgc ccccagctca gctcttactg
      181 cgggccgctc cgacggcggt ccatcctgtc agggactatg cggcgcaaga ttacaaggac
      241 gacgatgaca aggtggatga aatgaccaaa aagttcggca cgctcaccat tcacgacacc
      301 gaaaaggccg ccgaattcat ggctgagagg cccttccagt gtcgaatctg catgcgtaac
      361 ttcagtgacc gctccgccct gtcccgccac atccgcaccc acaccggcga gaagcctttt
      421 gcctgtgaca tttgtgggag gaaatttgcc cgctccgacg ccctggcccg ccataccaag
      481 atacacacgg gcagccaaaa gcccttccag tgtcgaatct gcatgcgtaa cttcagtcgc
      541 tccgacaccc tgtccgagca catccgcacc cacaccggcg agaagccttt tgcctgtgac
      601 atttgtggga ggaaatttgc ccaccactcc gcccgccgct gtcataccaa gatacacacg
      661 ggcgagaagc ccttccagtg tcgaatctgc atgcgtaagt ttgcccgctc cgacaacctg
      721 acccgccata ccaagataca cctgc
//
