LOCUS       sequence-106174-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mtZFN2G architecture bearing (+) FokI domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Gammage PA, Rorbach J, Vincent AI, Rebar EJ, Minczuk M
  TITLE     Mitochondrially targeted ZFNs for selective degradation of 
            pathogenic mitochondrial genomes bearing large-scale deletions or 
            point mutations.
  JOURNAL   EMBO Mol Med. 2014 Apr;6(4):458-66. doi: 10.1002/emmm.201303672. 
            Epub 2014 Feb 24.
  PUBMED    24567072
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/emmm.201303672"; journalName: "EMBO Mol Med"; date: 
            "2014-04"; volume: "6"; issue: "4"; pages: "458-66"
COMMENT     Sequence Label: pcDNA3.1(-)_mtZFN(+)2G
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             229..255
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             229..255
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaacg ggccctctag aatgttgggg tttgtgggtc gggtggccgc tgctccggcc
      121 tccggggcct tgcggagact caccccttca gcgtcgctgc ccccagctca gctcttactg
      181 cgggccgctc cgacggcggt ccatcctgtc agggactatg cggcgcaata cccctacgac
      241 gtgcccgact acgccgtgga tgaaatgacc aaaaagttcg gcacgctcac cattcacgac
      301 accgaaaagg ccgccgaatt catggctgag aggcccttcc agtgtcgaat ctgcatgcgt
      361 aacttcagtg accgctccgc cctgtcccgc cacatccgca cccacaccgg cgagaagcct
      421 tttgcctgtg acatttgtgg gaggaaattt gcccgctccg acgccctggc ccgccatacc
      481 aagatacaca cgggcagcca aaagcccttc cagtgtcgaa tctgcatgcg taacttcagt
      541 cgctccgaca ccctgtccga gcacatccgc acccacaccg gcgagaagcc ttttgcctgt
      601 gacatttgtg ggaggaaatt tgcccaccac tccgcccgcc gctgtcatac caagatacac
      661 acgggcgaga agcccttcca gtgtcgaatc tgcatgcgta agtttgcccg ctccgacaac
      721 ctgacccgcc ataccaagat acacctgc
//
