LOCUS       sequence-106188-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Alexeyev MF
  TITLE     The pKNOCK series of broad-host-range mobilizable suicide vectors 
            for gene knockout and targeted DNA insertion into the chromosome of 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 1999 May;26(5):824-6, 828.
  PUBMED    10337469
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1999-05"; volume: "26(5)"; pages: "824-6, 
            828"
COMMENT     Sequence Label: pKNOCK-Cm
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     oriT            223..332
                     /note="incP origin of transfer"
     rep_origin      503..891
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     924..940
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     924..940
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg
       61 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcggacgcg
      121 tcaattcgag ggggatcaat tccgtgatag gtgggctgcc cttcctggtt ggcttggttt
      181 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
      241 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
      301 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
      361 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
      421 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaacgga attgatccgg
      481 ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt
      541 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
      601 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
      661 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
      721 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
      781 cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt
      841 agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac atgggaattc
      901 ccctccaccg cggtggcggc cgctctagaa ctagtggatc cc
//
