LOCUS       sequence-106227-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA
  TITLE     Sequence- and structure-specific RNA processing by a CRISPR 
            endonuclease.
  JOURNAL   Science. 2010 Sep 10;329(5997):1355-8.
  PUBMED    20829488
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-09-10"; volume: "329(5997)"; pages: "1355-8"
COMMENT     Sequence Label: pHMGWA-Pa14Csy4H29A
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..52
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             62..625
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLAQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     protein_bind    complement(645..669)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             683..700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(753..771)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      767..814
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaagcaggc tccgcggccg ccgtactcga ggaaaacctg tattttcagg gctccttcac
       61 catggaccac tacctcgaca ttcgcttgcg accggacccg gaatttcccc cggcgcaact
      121 catgagcgtg ctcttcggca agctcgccca ggccctggtg gcacagggcg gggacaggat
      181 cggcgtgagc ttccccgacc tcgacgaaag ccgctcccgg ctgggcgagc gcctgcgcat
      241 tcatgcctcg gcggacgacc ttcgtgccct gctcgcccgg ccctggctgg aagggttgcg
      301 ggaccatctg caattcggag aaccggcagt cgtgcctcac cccacaccgt accgtcaggt
      361 cagtcgggtt caggcgaaaa gcaatccgga acgcctgcgg cggcggctca tgcgccggca
      421 cgatctgagt gaggaggagg ctcggaaacg cattcccgat acggtcgcga gagccttgga
      481 cctgcccttc gtcacgctac gcagccagag caccggacag cacttccgtc tcttcatccg
      541 ccacgggccg ttgcaggtga cggcagagga aggaggattc acctgttacg ggttgagcaa
      601 aggaggtttc gttccctggt tctgataaaa gggtgggcgc gccgacccag ctttcttgta
      661 caaagtggtg atgtacctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      781 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      841 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttt
//
