LOCUS       sequence-106338-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: tdminiSOG-PCNA-19
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             70..90
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..444
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             472..789
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagccaccat ggnatccaaa aaagaagaga
       61 aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtaccggtc
      121 gccaccatgg agaagagctt cgtgatcacc gacccccgcc tgcccgacaa ccccatcatc
      181 ttcgccagcg acggcttcct ggagctgacc gagtacagca gagaggagat cctgggccgc
      241 aacggccgct tcctgcaggg ccccgagacc gaccaggcca ccgtgcagaa gatccgcgac
      301 gccatccgcg accagcgcga gatcaccgtg cagctgatca actacaccaa gagcggcaag
      361 aagttctgga acctgctgca cctgcagccc atgcgcgacc agaagggcga gctgcagtac
      421 ttcatcggcg tgcagctgga cggctctggt ggcgggagtg gtggcgggtc catggagaaa
      481 agtttcgtga taactgatcc acggctgcca gacaatccca tcatcttcgc atccgatggc
      541 ttcctggagc tgaccgagta ttccagagag gagatcctgg gccgcaatgg ccgctttctg
      601 cagggaccag agacagacca ggccacagtg cagaagattc gcgatgccat tagagatcag
      661 cgcgagatta ccgtgcagct gataaactac acaaaaagcg ggaagaaatt ctggaacctc
      721 ctgcacctcc agcccatgag ggaccagaag ggtgagctcc agtatttcat cggagtgcag
      781 ctggatggat ccggagaagg gcaagggcaa gggcaagggc cgggccgcgg ctacgcgtat
      841 cgatccatgt tcgaggcgcg cctggtccag ggctccatcc tcaagaaggt gttggaggca
      901 ctcaaggacc tcatcancga ggcctgctgg gatattagct cca
//
