LOCUS       sequence-106343-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for TAL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-TAL1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..53
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttcactagtg attggaatcc gccaccatgg actacaagga cgacgatgac aagaccgagc
       61 ggccgccgag cgaggcggct cgcagtgacc cccagctaga gggacgggac gcggccgagg
      121 ccagcatggc ccccccgcac ctggtcctgc tgaacggcgt cgccaaggag acgagccgcg
      181 cggccgcagc ggagccccca gtcatcgaac tgggcgcgcg cggaggcccg gggggcggcc
      241 ctgccggtgg gggcggcgcc gcgagagact taaagggccg cgacgcggcg acggccgaag
      301 cgcgccatcg ggtgcccacc accgagctgt gcagacctcc cgggcccgcc ccggcccccg
      361 cgcccgcctc ggttacagcg gagctgcccg gcgacggccg catggtgcag ctgagtcctc
      421 ccgcgctggc tgcccccgcc gcccccggcc gcgcgctgct ctacagcctc agccagccgc
      481 tggcctctct cggcagcggg ttctttgggg agccggatgc cttccctatg ttcaccacca
      541 acaatcgagt gaagaggaga ccttccccct atgagatgga gattactgat ggtccccaca
      601 ccaaagttgt tcggcgtatc ttcaccaaca gcc
//
