LOCUS       sequence-106361-         370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_12
FEATURES             Location/Qualifiers
     source          1..370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(106..140)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     195..212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(236..293)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(321..348)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cgacgaaaac tacgcttaag ttaaccacat aaacataaat ggtacaacta atgtggcacc
       61 tgtaccaccc atagttacca cgaacgttat cgctctcatc gaactgctag cattatacct
      121 aggactgagc tagctgtcaa attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt
      241 tcggttttaa agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc
      301 tgccgctact agtatataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta
      361 gtagagagcg
//
