LOCUS       sequence-106363-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_11
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(120..154)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(250..307)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(335..362)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(378..449)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaggcctgca gcaaacgacg aaaactacgc ttaaaattaa ttccaataaa tgttttgtac
       61 ctctttctgt aggggcatct gggaatctgc aaaatccatg ttatcgctct catcgaactg
      121 ctagcattat acctaggact gagctagctg tcaaattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcataac
      241 gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct tgcccttttt
      301 tgccggactg cagctgccgc tactagtata taaacgcaga aaggcccacc cgaaggtgag
      361 ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      421 tctttcgact gagcctttcg ttttatttga tgcctggctc tagtattatt aagcaccggt
      481 ggagtgacga ccttcagcac gttcgtactg ttcaacgatg gtgtagtctt cgttgtggga
      541 ggtgatgtcc agtttgatgt cggttttgta agcacccggc agctgaaccg gttttttagc
      601 catgtaggtg gttttaactt cagcgtcgta gtgaccaccg tctttcagtt tcagacgcat
      661 tttgatttca cctttcagag caccgtcttc cgggtacata cgttcggtgg aagcttccca
      721 acccatggtt tttttctgca taaccggacc gtcggacggg aagttggtac cacgcagttt
      781 aactttgtag atgaactcac cgtcttgcag ggaggagtcc tgggtaacgg taacaacacc
      841 accgtc
//
