LOCUS       sequence-106371-         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_3
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(102..136)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     191..208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(232..289)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(317..344)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(360..431)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgacgaaaac tacgcttaaa taagatgcct ttttaccgac gagtatagtt gtacatgcca
       61 ttatcagtct cctttacaaa cgttatcgct ctcatcgaac tgctagcatt atacctagga
      121 ctgagctagc tgtcaaatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      181 gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta atcttttcgg
      241 ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac tgcagctgcc
      301 gctactagta tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag
      361 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
      421 cgttttattt gatgcctggc tctagtatta ttaagcaccg gtggagtgac gaccttcagc
      481 acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt ccag
//
