LOCUS       sequence-106373-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_2
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(123..157)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(253..310)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(338..365)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(381..452)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actatacaaa aggcctgcag caaacgacga aaactacgct taagacgagc tgagcagtat
       61 gtcgacggtc cggcgaagag ctactcccct tctgcgccgt ccgttatcgc tctcatcgaa
      121 ctgctagcat tatacctagg actgagctag ctgtcaaatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcctgcat
      241 aacgcgaagt aatcttttcg gttttaaaga aaaagggcag ggtggtgaca ccttgccctt
      301 ttttgccgga ctgcagctgc cgctactagt atataaacgc agaaaggccc acccgaaggt
      361 gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc
      421 cagtctttcg actgagcctt tcgttttatt tgatgcctgg ctctagtatt attaagcacc
      481 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg
      541 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
      601 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
      661 cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc
      721 ccaacccatg gttt
//
