LOCUS       sequence-106374-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(61..136)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(153..401)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(211..230)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(381..401)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    452..569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(867..911)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctgacgggca ccggagccaa ttcccactcc tttcaagacc tagctagcga attcaaaaaa
       61 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      121 tttctagctc taaaacagag acgtccgtct ccggtgtttc gtcctttcca caagatatat
      181 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
      241 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
      301 actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat
      361 cgtatatgca aatatgaagg aatcatggga aataggccct cggtacctta attaaccaaa
      421 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
      481 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      541 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga
      601 attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct
      661 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
      721 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
      781 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
      841 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
      901 tgttcattct tt
//
