LOCUS       sequence-106422-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_11
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(214..300)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(535..552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(749..768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctagttg aaatatgttt
      121 actaataaga ctttatgggt tgagagcgtc ttattagtaa acatataaac acagaggaca
      181 catgccctac ctccatgatc tctagggcgg cggatttgtc ctactcagga gagcgttcac
      241 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      301 atgccacgcg taccatggga ctagggccca tacccaaaaa aaaaccccgc ccctgacagg
      361 gcggggtttt ttttaggtag agacccacga ggcagacctc agcgctagcg gagtgtatac
      421 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
      481 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
      541 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
      601 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
      661 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
      721 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
      781 ctcgtgcgct ctcctgttcc tgcctttcgg t
//
