LOCUS       sequence-106544-        6872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: pBadcrtEBI_13
FEATURES             Location/Qualifiers
     source          1..6872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             36..44
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             2048..2056
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      complement(3767..3794)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3810..3881)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             3994..4653
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4060..4079)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4674..4768
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4857..5445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5346..5365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5690..6568)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(6595..6872,1..7)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     6802..6821
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tctccatacc cgtgaattcg atttttagga acagttaagg aggttaataa tggcattcga
       61 acaacgtatt gaagctgcta tggctgctgc tattgcacgt ggccagggta gcgaagctcc
      121 gtccaaactg gcaactgcgc tggactacgc tgtcacccct ggtggtgcgc gcattcgtcc
      181 gactctgctg ctgtctgtcg ccaccgcttg cggcgatgac cgtcctgccc tgagcgacgc
      241 ggctgcggtt gccctggaac tgatccattg cgcttctctg gtgcatgacg acctgccgtg
      301 cttcgacgac gctgaaatcc gtcgtggtaa gccaaccgtt catcgtgcat acagcgaacc
      361 gctggctatc ctgactggtg attccctgat cgttatgggt ttcgaagttc tggcgcgtgc
      421 ggcggctgat cagccacaac gtgccctgca actggtaacg gctctggcgg tacgcactgg
      481 tatgccgatg ggcatctgcg ctggtcaggg ttgggaaagc gaatctcaga tcaacctgtc
      541 tgcataccac cgtgccaaaa ctggtgccct gttcatcgcg gctactcaga tgggcgcaat
      601 cgcagcgggt tacgaggcgg aaccgtggga agaactgggt gcgcgtatcg gcgaagcgtt
      661 ccaggttgca gatgatctgc gtgacgcgct gtgcgatgcc gaaaccctgg gtaagccggc
      721 aggtcaggac gaaatccatg cacgtccgaa cgctgttcgc gagtatggtg tagaaggcgc
      781 ggctaaacgc ctgaaagaca tcctgggtgg cgcgatcgca tccatcccgt cctgtccagg
      841 tgaagcaatg ctggcagaga tggttcgtcg ctacgccgag aaaatcgttc cagctcaagt
      901 tgctgcacgc gtgtaagagt acaatagaaa ttaaaatcag gaggtcaaca atgattgctt
      961 ctgctgatct ggatgcgtgt cgtgaaatga ttcgtaccgg ctcctatagc tttcatgccg
     1021 cttctcgtct gctgccggaa cgcgtacgtg cgccgagcct ggcgctgtac gccttctgcc
     1081 gtgtagctga cgatgctgtg gatgaagctg ttaacgatgg ccagcgtgaa gaagatgctg
     1141 aagtgaaacg ccgcgcagta ctgagcctgc gtgatcgtct ggatctggtg tatggcggcc
     1201 gtccgcgtaa cgctcctgct gaccgcgcct ttgcggcggt cgtcgaagaa tttgaaatgc
     1261 cgcgtgctct gccggaagcg ctgctggaag gtctggcgtg ggatgcggta ggccgttcct
     1321 acgactcctt cagcggtgtt ctggattact ctgcgcgtgt tgccgcagcc gttggcgcga
     1381 tgatgtgtgt tctgatgcgc gttcgcgatc ctgatgtcct ggctcgtgcg tgcgatctgg
     1441 gtctggcgat gcaactgacc aacatcgctc gtgacgttgg cactgacgcc cgttctggtc
     1501 gcatctacct gccgcgcgac tggatggaag aggaaggcct gccggtggaa gagttcctgg
     1561 cacgcccggt ggttgatgat cgcatccgtg cagtcactca ccgcctgctg cgcgctgcgg
     1621 accgtctgta cctgcgttct gaggccggtg tgtgtggcct gccgctggct tgtcgtccgg
     1681 gcatttacgc ggcacgtcac atctatgctg gtattggtga tgaaatcgcg cgtaacggtt
     1741 atgattctgt tacccgtcgc gctttcacca ctcgtcgcca gaaactggtt tggctgggtc
     1801 tgtccgctac tcgcgctgct ctgagcccat tcggtccagg ttgtgcaacc ctgcacgccg
     1861 ctccggaacc agaagtcgca ttcctggtta acgcggcagc acgcgctcgt cctcagcgtg
     1921 gccgtagcga agctctgatt agcgttctgg ctcaactgga ggctcaggat cgtcagatca
     1981 gccgccagcg tctgggtaac cgtgcgaacc cgatctaact cgaagcttag attttaaata
     2041 acaatactaa ggaggtgcaa catgccatct atttctccag cctctgacgc tgatcgtgct
     2101 ctggttatcg gctctggcct gggcggcctg gccgctgcta tgcgtctggg tgcaaaaggt
     2161 tggcgtgtta ccgtaatcga taaactggac gttccgggtg gccgtggctc cagcatcacg
     2221 caggagggcc atcgttttga cctgggcccg accattgtta ctgtacctca aagcctgcgt
     2281 gacctgtgga aaacttgcgg tcgcgacttc gacgcagatg tggagctgaa accgattgac
     2341 ccattctacg aagttcgttg gccagatggc tctcacttta ccgttcgtca gtctactgaa
     2401 gctatgaaag ctgaagtagc acgcctgtct ccaggcgatg ttgcgggcta cgagaaattc
     2461 ctgaaggatt ccgagaaacg ttattggttc ggttatgagg acctgggtcg tcgctccatg
     2521 cacaaactgt gggatctgat caaagtcctg ccgaccttcg gcatgatgcg tgctgatcgt
     2581 tccgtctacc agcacgcggc cctgcgcgtc aaggatgaac gcctgcgcat ggcgctgtcc
     2641 ttccatcctc tgttcattgg tggtgatccg ttcaatgtga ccagcatgta catcctggta
     2701 tctcaactgg agaaagaatt tggtgtacat tacgcgatcg gcggtgtggc cgccatcgca
     2761 gccgcgatgg cgaaagtgat tgaaggtcag ggcggctcct tccgcatgaa cactgaagtg
     2821 gacgaaatcc tggttgaaaa aggcaccgct accggtgttc gcctggcgtc tggcgaagta
     2881 ctgcgcgctg gtctggttgt aagcaatgcg gacgcaggcc atacctacat gcgtctgctg
     2941 cgcaatcacc ctcgccgtcg ttggactgac gcccacgtta aatctcgccg ctggagcatg
     3001 ggcctgtttg tttggtactt tggcaccaaa ggtactaaag gcatgtggcc ggatgtgggt
     3061 catcacacca tcgtgaacgc tccgcgttac aaaggcctgg ttgaggacat cttcctgaaa
     3121 ggtaaactgg ccaaagacat gtccctgtac atccatcgtc catctatcac tgatccgacc
     3181 gttgcgccag aaggcgacga taccttctac gcgctgtccc cagtgccgca cctgaaacag
     3241 gcacagccgg tagactggca ggcggttgcg gagccgtacc gcgaaagcgt cctggaagtc
     3301 ctggaacaat ccatgccggg cattggtgaa cgcattggtc caagcctggt gttcacgcca
     3361 gaaactttcc gtgatcgtta cctgtccccg tggggtgcag gcttctctat cgaaccgcgc
     3421 atcctgcaat ctgcttggtt ccgtccgcat aatatctctg aagaagtcgc aaatctgttt
     3481 ctggttggtg cgggcaccca cccaggtgcc ggtgtaccgg gtgttattgg ctctgcggag
     3541 gtcatggcga aactggctcc ggatgcgccg cgtgcgcgcc gtgaagctga accggctgag
     3601 cgcctggcag cagaataacc ataacacaca cacacacaag gtcgtcacag caccggtgct
     3661 taataaagat cttcgacgtc tgctgtgtaa aaaaaaaccc cgcccctgac agggcggggt
     3721 tttttttcgc ggttgtgaat gccatatggc tgtgctgtgg tataaacgca gaaaggccca
     3781 cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa
     3841 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc ctaggttcac
     3901 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     3961 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     4021 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     4081 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     4141 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     4201 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     4261 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     4321 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     4381 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     4441 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     4501 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     4561 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     4621 cagtactgcg atgagtggca gggcggggcg taatttgata tcggtaccgc ttggactcct
     4681 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     4741 ccgccgggcg ttttttattg gtgagaatcc aagcacccgg gcatgaccaa aatcccttaa
     4801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4981 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac ctggatcctg
     5581 ataaatgatt tctgcagaaa acataatgtg cctgtcaaat ggacgaagca gggattctgc
     5641 aaaccctatg ctactccctc gagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     5701 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     5761 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     5821 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     5881 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     5941 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     6001 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     6061 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     6121 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     6181 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     6241 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     6301 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     6361 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     6421 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     6481 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     6541 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     6601 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     6661 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     6721 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     6781 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatctta
     6841 cctgacgctt tttatcgcaa ctctctactg tt
//
