LOCUS       sequence-106566-        5778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is designed as a bacterial oxidative stress sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5778)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: dps-RFP
FEATURES             Location/Qualifiers
     source          1..5778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     887..906
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     RBS             1432..1440
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1446..2123
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        2198..2232
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      3067..3153
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3236..3253)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3406..3492
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3584..3611
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3630..3721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3722..3790,3791..4582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3940..3959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4624..5079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4711..4730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctaggat catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      901 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      961 agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg tcaaatggac
     1021 gaagcaggga ttctgcaaac cctatgctac tccctcgagc cggatccgct acttttcctc
     1081 tacaccgtct ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga
     1141 gcagataaat aagaattgtt cttatcaata tatctaactc attgaatctt tattagtttt
     1201 gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat
     1261 agccggtgct atacttaatc tcgttaatta ctgggacata acatcaagag gatatgaaat
     1321 tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga
     1381 tgtctccgac agcgagaaaa aagcaacagt agaataatct agaaaaaaaa ataaggaggt
     1441 aaaaaatggc gagctctgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     1501 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     1561 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     1621 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     1681 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     1741 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     1801 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     1861 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     1921 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     1981 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     2041 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     2101 gtcgtcactc caccggtgct taataaggcc ggccgtgcta gtgtagaaat agccgccagt
     2161 tccgctggcg gcattttaac tttctttagt actagtattg acagctagct cagtcctagg
     2221 tactgtgcta gcaccccaat cgacgtcata ctagaatcgg gtcacggagg tacaataata
     2281 atgcgtaaac tcgaggaact tttcacgggg gtcgtcccaa ttctggtaga attagacggt
     2341 gatgtaaacg ggcacaagtt tagcgtcagc ggagagggcg agggggacgc cacctacggt
     2401 aaacttaccc ttaagtttat ttgcacgacg ggcaaattac ccgttccgtg gccaacgctc
     2461 gtcactactt tcggctacgg cgttcagtgc ttcgcgagat atccggatca catgaagcag
     2521 cacgatttct tcaagtcggc catgccagag ggttacgttc aggagagaac tatatttttc
     2581 aaggacgacg gcaattacaa gacacgcgcc gaagtcaagt tcgaaggcga taccctggtt
     2641 aaccgtatcg agttaaaagg tatcgatttt aaagaagacg gaaacattct cggtcacaaa
     2701 ctcgaataca actataacag ccataatgtc tacattatgg ctgacaaaca gaagaacggc
     2761 attaaggtta acttcaaaat tcgccacaac atcgaagacg gtagcgtcca actagcagac
     2821 cattatcaac aaaatactcc tatcggcgac ggcccagtcc ttttaccaga caaccattac
     2881 cttagcacac aaagcgccct tagcaaagat cccaacgaga agcgcgacca catggtcctt
     2941 ctcgaatttg tcacagcggc agggatcacc cacggcatgg acgaactcta caaataataa
     3001 ttaattaaaa aaaaaaaccc cgcccctgac agggcggggt ttttttttct aatatgcggc
     3061 cgccatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3121 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagagcat gcgcgttcgg
     3181 ctgcggcgag cggtatcagg cttggctgtt ttggcggatg agagaagatt ttcagcctga
     3241 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     3301 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     3361 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     3421 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3481 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     3541 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     3601 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     3661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagac
     3721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3901 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3961 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4021 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4081 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4141 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4261 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4561 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4621 tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4801 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4921 gtggactctt gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt
     4981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5041 ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc
     5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5401 gctctgctaa tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg
     5461 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
     5521 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact
     5581 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
     5641 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     5701 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5761 gtataatatt tgcccatg
//
