LOCUS       sequence-106584-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lenti sgRNA cloning backbone with MS2 loops at 
            tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains 
            BsmBI sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti sgRNA(MS2)_zeo backbone
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..391
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(469..489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggtgtcgtga tgtacaatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
       61 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
      121 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
      181 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
      241 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
      301 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
      361 cgtgcacttc gtggccgagg agcaggactg agaattcgat atcaagctta tcggtaatca
      421 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
      481 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
      541 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
      601 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
      661 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
      721 cacggcggaa ctcatcgccg cctgccttg
//
