LOCUS       sequence-106820-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating cyan fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Cg
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(254..256,257..259,260..772)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(299..320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(560..579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatagg
      241 gcccgggatc ctgatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      301 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      361 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      421 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      481 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      541 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      601 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      661 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      721 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
      781 cgtgcagctc gccgaccact accagca
//
