LOCUS       sequence-106863-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB5
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgaattcac catgggtaag
      181 cctatcccta accctctcct cggtctcgat tctacgggat ccaaaacctc gagaaccatg
      241 gacatcgcca tccaccaccc ctggatccgc cgccccttct ttcctttcca ctcccccagc
      301 cgcctctttg accagttctt cggagagcac ctgttggagt ctgatctttt cccgacgtct
      361 acttccctga gtcccttcta ccttcggcca ccctccttcc tgcgggcacc cagctggttt
      421 gacactggac tctcagagat gcgcctggag aaggacaggt tctctgtcaa cctggatgtg
      481 aagcacttct ccccagagga actcaaagtt aaggtgttgg gagatgtgat tgaggtgcat
      541 ggaaaacatg aagagcgcca ggatgaacat ggtttcatct ccagggagtt ccacaggaaa
      601 taccggatcc cagctgatgt agaccctctc accattactt catccctgtc atctgatggg
      661 gtcctcactg tgaatggacc aaggaaacag gtctctggcc ctgagcgcac cattcccatc
      721 acccgtgaag agaagcctgc tgtcaccgca gcccccaaga aatagatgcc ctttcttgaa
      781 ttgcatttt
//
