LOCUS       sequence-106867-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with bGpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_bGpA
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..95)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    141..196
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(195..214)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        551..655
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
       61 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      121 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      181 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
      241 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
      301 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
      361 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
      421 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
      481 actactccca gtcatagctg tccctcttct cttatgaact cgactgcgtc gaccaattcg
      541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      721 tgctcaccca g
//
