LOCUS       sequence-106896-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP in T. cruzi. The NheI-linearized form of this 
            plasmid could be efficiently integrated in to T. cruzi genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
  TITLE     CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in 
            Trypanosoma cruzi.
  JOURNAL   MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
  PUBMED    25550322
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-12-30"; volume: "6(1). pii: e02097-14. doi"; pages: " 
            10.1128/mBio.02097-14"
COMMENT     Sequence Label: pTrex-n-eGFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtacccaca cggctagcat actctagcgg
       61 catatacaca ggtgggctat aatgcccagt attgttcgaa aaagtccaaa gaacaacaac
      121 actacgacgc ataatgcaca aacactaaac tacaccttaa acaccatcag tgaagaaaaa
      181 gaaaaaaaaa gaaaaacagc ttaaatcctt aaaaaaaaaa aatcacacta aattaaaaca
      241 gtcagttata aacaaaaaga aactgaacaa ccaaaatctt ccaataacgc aaagttgtaa
      301 aaaattacca caagctgcat catacaccca atcaaatcat ctacattttc cttgcaatac
      361 cccaaatcct cgtacattga ttaaacatta tttcactcat ccttccgcat cattaaattg
      421 ctgctttttg tttcccaaag atgaccataa atataatttc aaaaaaaaaa aagaaaaaaa
      481 agttatatat ttttttgttg ttgtccttaa agggaaaagt aaaattgttt tgctcttgac
      541 actgtgtcac ttggatcgtg gcagcaccgg taacgatcat cacgggacac gcatcgtgta
      601 ttataagatc cctattcgca aaagaaagac ctgaagtcat cgccacgcct acaaccttgc
      661 cgaacgatcc ttcgaggcca tgtggcgtac aaggtccacc acgcggtggg agtatcccca
      721 ctcgttgtcg taccacgaca caatcttgaa gaagcggcgc tccttcggca ggttgtgact
      781 aagattcctc tttgagctgc ccgagcatcc aaagatcaaa ttcagaagac ttgctggcaa
      841 tcctgagagt ggctaacgcc tgtgcacgga tcgatcc
//
