LOCUS       sequence-106904-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter containing a fragment of the 3'UTR
            of SPRED1 containing a miR-206 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg
       61 cggaaagtcc aaattgctcg agtgatgaaa gctgcgcact agtgagggag ctcataggcc
      121 ggcatagacg cgtccgattt aactaaatgt aaggtacgaa ttattacata ttaaactttt
      181 cttccccttc ctagttctga agtagatata tatatatata tatctactgt cacattccat
      241 atattttgaa tatttaactc atctagttaa taatgttttt attccatgcg aatgatttga
      301 tattttcatc cttatttctc tttggctaca atttatattg agttatatct gtacattctg
      361 gtaatctaaa atccttaaaa atactctaat agccttgagt gaccaacttt ttttttaaag
      421 cacagatgta attgtctaat gttctgatgg gaacgtaaca cttattttta tataaaaaga
      481 gactgagtaa acaaacatta tagaaaaaaa gtgaagtttt ttagttgttt tttgtggtat
      541 tcaaccagca agttgttttc tttcagagtt tcctccttca aaaagttata ttgcatttac
      601 aaatgtttta caaggcagaa agtttgactg gatagttagt gtaaaagctt catgttgaga
      661 tcttcacgta tcattctgct aaaccagaat atgttcagct gtgttact
//
