LOCUS       sequence-106910-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter encoding a mutated fragment of the
            SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa
       61 ttgctcgagt gatgaaagct gcgcactagt gagggagctc ataggccggc atagacgcgt
      121 tgaaaaactg tttaactcat gtcataatgt gtctcctcag atatagttaa ctagcttgat
      181 agttttgagt atataatgta aataatacat aataagtttg taaccatttc tgttacttca
      241 tacccgtgta attggacata gcagatgatt atcataagaa tttgttttaa tggttttttt
      301 ccctcctgga attacctgat taaacctgtc atgaattata aaaggacttt ttcttattct
      361 cctagagctt atcaaattat cacataagag ataattactg gaggagaaag taaaaatgga
      421 agcttttgat aattagctct gttctttaaa agtatacttt taaaccgaaa gttcacatat
      481 agttcatctt aataacatat ttattcatcc ttaattgtat tcagattttt ttttaagtct
      541 ctaagctaat aatgttatat ttattggttt ggtaacatgt tgcagctaga tcaatgacct
      601 taagtggcaa ttgtttaacc caggactact gattttttta tattcaagtc agtttcattg
      661 ttttacttaa ctcgccatat aacc
//
