LOCUS       sequence-106913-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Splicing reporter control (no intron) with elements to shorten the 
            half-life of the luciferase protein as well as the luciferase mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G
  TITLE     Rapid-response splicing reporter screens identify differential 
            regulators of constitutive and alternative splicing.
  JOURNAL   Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. 
            Epub 2010 Feb 1.
  PUBMED    20123975
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01301-09"; journalName: "Mol Cell Biol"; date: 
            "2010-04"; volume: "30"; issue: "7"; pages: "1718-28"
COMMENT     Sequence Label: CMV-LUC2CP/ARE
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..118
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             122..241
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    314..435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(325..344)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     379..398
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(652..672)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc
       61 cgtgaattct gcttgcaaga actggttcag tagcttaagc cactttgtga tccaccttaa
      121 cagccacggc ttccctcccg aggtggagga gcaggccgcc ggcaccctgc ccatgagctg
      181 cgcccaggag agcggcatgg atagacaccc tgctgcttgc gccagcgcca ggatcaacgt
      241 ctaactgcag tctagattta tttatttatt tatttctaga gtcggggcgg ccggccgctt
      301 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      361 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      421 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      481 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
      541 ccgtttgcgt attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct
      601 ctgaaagagg aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt
      661 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      721 atctcaatta gtcagcaacc a
//
