LOCUS       sequence-106921-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC84
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggaggagcag ccccagatgc aagacgccga
      181 cgagcccgcg gactccggag gggaaggccg ggcaggcggg ccaccgcagg tcgccggcgc
      241 ccaggcggcg tgcagcgagg accgcatgac cctgctcctc aggctgagag cacagacaaa
      301 acaacaactc ttagaatata aatcaatggt tgatgcaagt gaagaaaaaa ctccagaaca
      361 aattatgcaa gaaaagcaaa tcgaagctaa aattgaagac ctggaaaatg aaattgaaga
      421 ggtaaaagtt gcttttgaga taaaaaagct tgcattagac aggatgagac tttcaactgc
      481 acttaaaaaa aacctggaga aaattagcag acagtctagt gtgctcatgg ataacatgaa
      541 acacctatta gagctaaata aattaataat gaaatcacag caggaatctt gggatttaga
      601 ggaaaaactg cttgatatta gaaagaagag attgcaatta aaacaagctt cagaaagtaa
      661 gcttttagaa atacagactg aaaagaacaa acagaagatt gatttggaca gtatggaaaa
      721 ctcagagagg ataaagatca tacgacaaaa cctacagatg gagat
//
