LOCUS       sequence-106959-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-T for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG113
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggcagaccac aaccctgaca gcgattctac
      181 acctcgtaca ctgctgcgtc gtgtgctgga tactgctgat cctcgtactc ctcgtcgtcc
      241 tcgtagcgct cgtgctggtg ctcgccgtgc cctgctggaa acagcctctc ctcgtaaact
      301 gagtggccag acccgtacta ttgcccgtgg gcgttcacat ggagcccgct cggtcggtcg
      361 tagtgctcac attcaagcct cgggacacct ggaagaacaa acccctcgta ccctgctgaa
      421 aaacatcctg ctgactgccc ctgaaagctc tattctgatg ccggaaagcg tcgttaaacc
      481 ggttcctgct cctcaagccg ttcaaccttc tcgtcaagag tcctcctgtg gatctctgga
      541 gctgcaactg cctgaactgg agcctcctac aacactggca ccggggctgc tggcgcctgg
      601 tcgtcgtaaa caacgtctgc gtctgagcgt gtttcaacaa ggtgttgatc agggcctgag
      661 tctgtctcaa gaacctcaag gcaacgctga tgcttctagt ctgacccgtt ctctgaacct
      721 gacctttgct actccactgc aacctcaaag cgtccaacgt cctggactgg ctcgccg
//
