LOCUS       sequence-106986-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC79
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggcggggttt cctgagttgg tggtccttga
      181 ccctccatgg gacaaggagc tcgcggctgg cacagagagc caggccttgg tctccgccac
      241 tccccgagaa gactttcggg tgcgctgcac ctcgaagcgg gctgtgaccg aaatgctaca
      301 actgtgcggc cgcttcgtgc aaaagctcgg ggacgctctg ccggaggaga ttcgggagcc
      361 cgctctgcga gatgcgcagt ggacttttga atcagctgtg caagagaata tcagcattaa
      421 tgggcaagca tggcaggaag cttcagataa ttgttttatg gattctgaca tcaaagtact
      481 tgaagatcag tttgatgaaa tcatagtaga tatagccaca aaacgtaagc agtatcccag
      541 aaagatcctg gaatgtgtca tcaaaaccat aaaagcaaaa caagaaattc tgaagcagta
      601 ccaccctgtt gtacatccac tggacctaaa atatgaccct gatccagccc ctcatatgga
      661 aaatttgaaa tgcagagggg aaacagtagc aaaggagatc agtgaagcca tgaagtcctt
      721 gcctgcatta attgaacaag gagagggatt ttcccaagtt ctcaggatgc agcctgttat
//
