LOCUS       sequence-107-exp        4491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_79
FEATURES             Location/Qualifiers
     source          1..4491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     944..964
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1931..1953)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2053..2072
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2150..2168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2236..2340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2341..2409,2410..3201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2559..2578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3372..3960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3861..3880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4114..4131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4248..4269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4284..4289,4290..4307,4308..4314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4322..4338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4327..4349
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4346..4362
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4346..4362
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      121 gggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
      361 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
      421 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      481 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
      541 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
      601 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      661 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      721 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
      781 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      841 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      901 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      961 taacagctgc tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt
     1021 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
     1081 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
     1141 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     1201 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     1261 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     1321 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     1381 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     1441 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     1501 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     1561 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     1621 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     1681 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     1741 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     1801 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
     1861 cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg
     1921 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     1981 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2041 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     2101 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata
     2161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2341 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2461 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2521 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2581 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2641 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2701 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2881 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2941 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3001 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3181 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3481 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3721 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     3781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4081 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4141 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4201 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4261 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4321 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg
     4381 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa
     4441 tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
