LOCUS       sequence-107070-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A with K13R tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K13R
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(589..606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctgg ctagcgttta aacttaagct taccatggac tacaaggacg acgatgacaa
      121 ggtacctagg atccagtcgg gacgtggcaa gcagggcggc aaagctcgcg ccagggccaa
      181 gacccgctct tctcgggccg ggcttcagtt tcccgtaggc cgagtgcatc gcctgctccg
      241 caaaggcaac tatgcggagc gggtcggtgc tggagcgccg gtgtacctgg cggcggtgct
      301 ggagtacctg accgccgaga tcctggagct ggctggcaac gcggcccgcg acaacaagaa
      361 gactcgcatc atcccgcgtc acctccagct ggccatccgc aacgatgagg agctcaacaa
      421 gcttctgggc aaagtcacca tcgcacaggg tggcgtcctg cccaacatcc aggccgtgct
      481 actgcccaag aagaccgaga gccaccacaa ggccaagggc aagtaacaga tatccagcac
      541 agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
      601 cttctagttg ccagccatct gttg
//
