LOCUS       sequence-107078-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A (with multiple Lysine to Arginine point 
            mutations) tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K13-15-118-119R
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(436..459)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(500..519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(501..519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(564..663)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(593..613)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatctg ttacttgccc ttggccttgt ggtggctctc
       61 ggtcctcctg ggcagtagca cggcctggat gttgggcagg acgccaccct gtgcgatggt
      121 gactttgccc agaagcttgt tgagctcctc atcgttgcgg atggccagct ggaggtgacg
      181 cgggatgatg cgagtcttct tgttgtcgcg ggccgcgttg ccagccagct ccaggatctc
      241 ggcggtcagg tactccagca ccgccgccag gtacaccggc gctccagcac cgacccgctc
      301 cgcatagttg cctttgcgga gcaggcgatg cactcggcct acgggaaact gaagcccggc
      361 ccgagaagag cgggtcctgg ccctggcgcg agctttgccg ccctgcttgc cacgtcccga
      421 ctggatccta ggtaccttgt catcgtcgtc cttgtagtcc atggtaagct taagtttaaa
      481 cgctagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca
      541 gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta
      601 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      661 tggtg
//
