LOCUS       sequence-107092-        7313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid of HA-tagged human Neuroligin 3 harboring an 
            R451C mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Chih B, Afridi SK, Clark L, Scheiffele P
  TITLE     Disorder-associated mutations lead to functional inactivation of 
            neuroligins.
  JOURNAL   Hum Mol Genet. 2004 Jul 15;13(14):1471-7. Epub 2004 May 18.
  PUBMED    15150161
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet."; date: "2004-07-15"; volume: "13(14)"; pages: "1471-7. Epub 
            2004 May 18"
COMMENT     Sequence Label: pCAG-HA-NLGN3 R451C
FEATURES             Location/Qualifiers
     source          1..7313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1812..1838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4317..4336)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4382..4437
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4436..4455)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4798..4814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4798..4814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4811..4833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4822..4838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4846..4852,4853..4870,4871..4876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4891..4912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4971..5166
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5017..5152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5172..5306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5209..5228)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5263..5282
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5374..5391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5545..6133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5625..5644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6304..7095,7096..7164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6927..6946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7165..7269)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7300..7313,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc accatggctg
     1741 ctggctgcct gctggcctgg acgctgacac tttggcaggc gtggctgatc ctgatcgggc
     1801 cctcgtcgga gtacccatac gacgtcccag actacgctgg taccgcaccc acagtcaaca
     1861 ctcactttgg gaagctaagg ggtgcccgag taccactgcc cagtgagatc ctggggcctg
     1921 tggaccaata cctgggggtg ccctacgcag ctcccccgat cggcgagaaa cgtttcctgc
     1981 cccctgaacc acccccatac tggtcgggca tccggaacgc cacacacttt ccaccagtgt
     2041 gcccccagaa catccacaca gctgtgcccg aagtcatgct gccggtctgg ttcactgcca
     2101 acttggatat cgtcgctact tacatccagg agcccaacga agactgtctc tacctgaacg
     2161 tctatgtgcc gacggaggat ggatccggcg ctaagaaaca gggcgaggac ttagcggata
     2221 atgacgggga tgaagatgaa gacatccggg acagtggtgc taaacccgtc atggtctaca
     2281 tccacggagg ctcttacatg gaagggacag gcaacatgat tgatggcagc atcctcgcca
     2341 gttatggcaa tgtcatcgtc atcaccctca actatcgggt tggagtgcta ggtttcctga
     2401 gtactggaga tcaggctgcc aagggcaact atgggctcct tgaccagatc caggccctcc
     2461 gctgggtgag cgagaatatt gccttcttcg ggggagaccc ccgccggatc actgtctttg
     2521 gctcgggcat tggtgcatcc tgcgtcagcc tcctaacgtt gtcacatcac tcagagggac
     2581 ttttccagag agccatcatc caaagtggct ctgctctgtc cagctgggct gtgaactacc
     2641 aaccagtgaa gtacaccagc ctgctggcag acaaagtggg ctgtaatgtg ctggacaccg
     2701 tggatatggt ggactgtctt cggcaaaaga gtgccaagga gctggtagag caggacatcc
     2761 agccagcccg ctaccacgtg gcctttggcc ctgtgattga tggtgatgtc attcctgatg
     2821 accctgagat cctcatggag cagggcgagt tcctcaacta tgacatcatg ctaggtgtca
     2881 accagggcga gggtctcaag tttgtggaag gggtggtgga ccctgaggat ggtgtctctg
     2941 gcactgactt tgactattcc gtctccaatt ttgtggacaa tctgtatggc tatcctgagg
     3001 gtaaggacac cctgcgagag accatcaagt tcatgtatac agactgggca gaccgtgaca
     3061 accctgagac ccgctgtaaa acactggtgg cactcttcac tgaccaccag tgggtggagc
     3121 cctcagtggt gacagccgat ctgcatgccc gctacggctc gcctacctac ttctacgcct
     3181 tctatcatca ctgccagagc ctcatgaagc ctgcttggtc agatgcagct catggggatg
     3241 aagtacccta tgtttttggg gttcctatgg taggccccac tgaccttttc ccctgcaact
     3301 tctccaagaa tgatgttatg ctcagtgctg tcgtcatgac ctattggacc aactttgcca
     3361 agactgggga tcccaacaag ccggtccccc aggacaccaa gttcattcac accaaggcca
     3421 accgctttga ggaagtggcc tggtccaaat acaatccccg agaccagctc taccttcaca
     3481 tcgggctgaa accaagggtc cgagatcatt accgggccac taaggtggcc ttttggaaac
     3541 atctggtgcc gcacctatac aacctgcatg acatgttcca ctatacgtcc accaccacca
     3601 aagtgccgcc tccggatacc acccacagct cccacatcac ccgcaggccc aatggcaaga
     3661 cctggagcac caagcggcca gccatctcac ctgcctacag caacgagaat gcccaggggt
     3721 cctggaacgg ggaccaggat gcagggccac tcctggtgga gaaccctcgt gactactcca
     3781 ctgaattaag tgtcaccatc gccgtggggg cctccatcct gttccttaac gttctggcct
     3841 tcgctgccct ctactaccgt aaggacaaac ggcgccagga gcccctgcgg cagcctagcc
     3901 ctcagcgggg agcctgggcc ccggagttgg gagctgctcc agaggaggag ctggcagcat
     3961 tacaactggg ccccacccac cacgagtgtg aggccagtcc cccccatgac acgctgcgcc
     4021 tcactgcatt gcccgactac accctgaccc tgcggcgctc cccggatgac atcccactca
     4081 tgacccccaa caccatcact atgatcccca actccctggt agggctgcag acattgcacc
     4141 cctataacac ctttgccgca gggttcaaca gtaccgggct gccccactca cactccacta
     4201 cccgggtata gctcgaggta cccccgggga gctcaattca ctcctcaggt gcaggctgcc
     4261 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     4321 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     4381 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     4441 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     4501 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     4561 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     4621 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     4681 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     4741 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     4801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     4981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5161 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5281 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     5341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7261 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttg
//
