LOCUS       sequence-107157-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenoviral vector for XBP1u expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Zeng L, Zampetaki A, Margariti A, Pepe AE, Alam S, Martin D, Xiao Q,
            Wang W, Jin ZG, Cockerill G, Mori K, Li YS, Hu Y, Chien S, Xu Q
  TITLE     Sustained activation of XBP1 splicing leads to endothelial apoptosis
            and atherosclerosis development in response to disturbed flow.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 May 19;106(20):8326-31. doi: 
            10.1073/pnas.0903197106. Epub 2009 May 1.
  PUBMED    19416856
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903197106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-05-19- 19"; volume: "106"; issue: "20"; pages: "8326-31"
COMMENT     Sequence Label: Ad-Flag-XBP1u
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagca
       61 tggactacaa ggacgacgat gacaaggaat tcagatctgg taccctggtg gtggtggcag
      121 ccgcgccgaa cccggccgac gggaccccta aagttctgct tctgtcgggg cagcccgcct
      181 ccgccgccgg agccccggcc ggccaggccc tgccgctcat ggtgccagcc cagagagggg
      241 ccagcccgga ggcagcgagc ggggggctgc cccaggcgcg caagcgacag cgcctcacgc
      301 acctgagccc cgaggagaag gcgctgagga ggaaactgaa aaacagagta gcagctcaga
      361 ctgccagaga tcgaaagaag gctcgaatga gtgagctgga acagcaagtg gtagatttag
      421 aagaagagaa ccaaaaactt ttgctagaaa atcagctttt acgagagaaa actcatggcc
      481 ttgtagttga gaaccaggag ttaagacagc gcttggggat ggatgccctg gttgctgaag
      541 aggaggcgga agccaagggg aatgaagtga ggccagtggc cgggtctgct gagtccgcag
      601 cactcagact acgtgcacct ctgcagcagg tgcaggccca gttgtcaccc ctccagaaca
      661 tct
//
