LOCUS       sequence-107169-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as
            well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites 
            of Int7. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pCas_5+7_gfp
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      137..208
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      224..251
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      279..336
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     promoter        432..466
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
       61 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      121 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      181 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      241 gcctttctgc gtttatatac tagtagcggc agctgcagtc cggcaaaaaa gggcaaggtg
      301 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
      361 tcgctcactg actcactgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      421 aaggcggtaa tttgacagct agctcagtcc taggtataat gctagcagtt cgatgagagc
      481 gataacgagc gccggatcag ggagtggacg acctgggagc gctacacgct gtggctgcgg
      541 tcggtgctta tttcagggtg taatccaggg tgattttttt cagcttgcct ttctcgcgat
      601 aaatcacaat cttttcgaag atgctgaaca gcagctcttt tttggtaaag tcgttggcct
      661 gctcaaactg ctgtttaaaa tcgatcattt tgctcaggcg gttatatttc tcttcaaccg
      721 gttctttatc ttcttccact tcgctcagct tgctatagat ttcttcttct tttttgttca
      781 gttcgttaat tttggcaaac agttcttctt tcggaatcgg gttacgatca tccatataca
      841 gatcgtacca tttctctttc tggcgtttga ttt
//
