LOCUS       sequence-107197-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episome vector for Phaeodactylum tricornutum (pUC-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings 
            CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, 
            Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter 
            JC, Allen AE, Dupont CL, Weyman PD
  TITLE     Designer diatom episomes delivered by bacterial conjugation.
  JOURNAL   Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925.
  PUBMED    25897682
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-04-21"; volume: "6:6925. doi"; pages: " 
            10.1038/ncomms7925"
COMMENT     Sequence Label: pPtPuc3
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(178..364)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 tatacctgtg tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta ctgcttctgc
       61 ctctttttct gggaagatcg agtgctctat cgctagggga ccacccttta aagagatcgc
      121 aatctgaatc ttggtttcat ttgtaatacg ctttactagg gctttctgct ctgtcatctt
      181 tgccttcgtt tatcttgcct gctcattttt tagtatattc ttcgaagaaa tcacattact
      241 ttatataatg tataattcat tatgtgataa tgccaatcgc taagaaaaaa aaagagtcat
      301 ccgctaggtg gaaaaaaaaa atgaaaatca ttaccgaggc ataaaaaaat atagagtgta
      361 ctaggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
      421 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
      481 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
      541 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
      601 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
      661 acagacaaga tgaaacaatt cggcattaat acctgagagc a
//
