LOCUS       sequence-107243-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCP-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC504
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             546..695
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
       61 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact atctcatata
      121 caatgtctct cgagatgccc aaaaagaaaa gaaaagtggg tagtatgtcc aaaaccatcg
      181 ttctttcggt cggcgaggct actcgcactc tgactgagat ccagtccacc gcagaccgtc
      241 agatcttcga agagaaggtc gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc
      301 gtcaaaacgg agccaagacc gcgtatcgcg tcaacctaaa actggatcag gcggacgtcg
      361 ttgattccgg acttccgaaa gtgcgctaca ctcaggtatg gtcgcacgac gtgacaatcg
      421 ttgcgaatag caccgaggcc tcgcgcaaat cgttgtacga tttgaccaag tccctcgtcg
      481 cgacctcgca ggtcgaagat cttgtcgtca accttgtgcc gctgggccgt ggctccggac
      541 gagccgatgc tctcgacgat tttgacctcg atatgctggg atccgacgct ctcgatgact
      601 tcgaccttga catgctgggg agtgacgctc tggacgactt cgatctcgac atgctgggct
      661 ccgacgccct ggatgacttc gatctggaca tgctcatcaa ctgagc
//
